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94
Thermo Fisher sjcl5
Recombinant proteins of SjCL1-5 were resolved in 12% SDS-PAGE and stained with Coomassie brilliant blue (A), and analyzed by western blot using a mouse monoclonal antibody against the His-tag (B), or a mixture of serum samples (in equal volume) from 6 S. japonicum -infected BALB/c mice (C) and 5 S. japonicum -infected rabbits (D) collected at 42 dpi, respectively. Lane M: Marker, Lane 1: SjCL1, Lane 2: SjCL2, Lane 3: SjCL3, Lane 4: SjCL4, Lane 5: <t>SjCL5.</t>
Sjcl5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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441100 depositor resolution determination method fsc  (Thermo Fisher)
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Thermo Fisher 441100 depositor resolution determination method fsc
Recombinant proteins of SjCL1-5 were resolved in 12% SDS-PAGE and stained with Coomassie brilliant blue (A), and analyzed by western blot using a mouse monoclonal antibody against the His-tag (B), or a mixture of serum samples (in equal volume) from 6 S. japonicum -infected BALB/c mice (C) and 5 S. japonicum -infected rabbits (D) collected at 42 dpi, respectively. Lane M: Marker, Lane 1: SjCL1, Lane 2: SjCL2, Lane 3: SjCL3, Lane 4: SjCL4, Lane 5: <t>SjCL5.</t>
441100 Depositor Resolution Determination Method Fsc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dialysis  (SERVA Electrophoresis)
92
SERVA Electrophoresis dialysis
Recombinant proteins of SjCL1-5 were resolved in 12% SDS-PAGE and stained with Coomassie brilliant blue (A), and analyzed by western blot using a mouse monoclonal antibody against the His-tag (B), or a mixture of serum samples (in equal volume) from 6 S. japonicum -infected BALB/c mice (C) and 5 S. japonicum -infected rabbits (D) collected at 42 dpi, respectively. Lane M: Marker, Lane 1: SjCL1, Lane 2: SjCL2, Lane 3: SjCL3, Lane 4: SjCL4, Lane 5: <t>SjCL5.</t>
Dialysis, supplied by SERVA Electrophoresis, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho ets2 invitrogen 44 1105g  (Thermo Fisher)
94
Thermo Fisher phospho ets2 invitrogen 44 1105g
Recombinant proteins of SjCL1-5 were resolved in 12% SDS-PAGE and stained with Coomassie brilliant blue (A), and analyzed by western blot using a mouse monoclonal antibody against the His-tag (B), or a mixture of serum samples (in equal volume) from 6 S. japonicum -infected BALB/c mice (C) and 5 S. japonicum -infected rabbits (D) collected at 42 dpi, respectively. Lane M: Marker, Lane 1: SjCL1, Lane 2: SjCL2, Lane 3: SjCL3, Lane 4: SjCL4, Lane 5: <t>SjCL5.</t>
Phospho Ets2 Invitrogen 44 1105g, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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visking dialysis tubing  (SERVA Electrophoresis)
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SERVA Electrophoresis visking dialysis tubing
Recombinant proteins of SjCL1-5 were resolved in 12% SDS-PAGE and stained with Coomassie brilliant blue (A), and analyzed by western blot using a mouse monoclonal antibody against the His-tag (B), or a mixture of serum samples (in equal volume) from 6 S. japonicum -infected BALB/c mice (C) and 5 S. japonicum -infected rabbits (D) collected at 42 dpi, respectively. Lane M: Marker, Lane 1: SjCL1, Lane 2: SjCL2, Lane 3: SjCL3, Lane 4: SjCL4, Lane 5: <t>SjCL5.</t>
Visking Dialysis Tubing, supplied by SERVA Electrophoresis, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho ets2  (Thermo Fisher)
94
Thermo Fisher anti phospho ets2
Recombinant proteins of SjCL1-5 were resolved in 12% SDS-PAGE and stained with Coomassie brilliant blue (A), and analyzed by western blot using a mouse monoclonal antibody against the His-tag (B), or a mixture of serum samples (in equal volume) from 6 S. japonicum -infected BALB/c mice (C) and 5 S. japonicum -infected rabbits (D) collected at 42 dpi, respectively. Lane M: Marker, Lane 1: SjCL1, Lane 2: SjCL2, Lane 3: SjCL3, Lane 4: SjCL4, Lane 5: <t>SjCL5.</t>
Anti Phospho Ets2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1104g  (Thermo Fisher)
94
Thermo Fisher 1104g
Recombinant proteins of SjCL1-5 were resolved in 12% SDS-PAGE and stained with Coomassie brilliant blue (A), and analyzed by western blot using a mouse monoclonal antibody against the His-tag (B), or a mixture of serum samples (in equal volume) from 6 S. japonicum -infected BALB/c mice (C) and 5 S. japonicum -infected rabbits (D) collected at 42 dpi, respectively. Lane M: Marker, Lane 1: SjCL1, Lane 2: SjCL2, Lane 3: SjCL3, Lane 4: SjCL4, Lane 5: <t>SjCL5.</t>
1104g, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p ets2 thr72  (Thermo Fisher)
94
Thermo Fisher p ets2 thr72
Recombinant proteins of SjCL1-5 were resolved in 12% SDS-PAGE and stained with Coomassie brilliant blue (A), and analyzed by western blot using a mouse monoclonal antibody against the His-tag (B), or a mixture of serum samples (in equal volume) from 6 S. japonicum -infected BALB/c mice (C) and 5 S. japonicum -infected rabbits (D) collected at 42 dpi, respectively. Lane M: Marker, Lane 1: SjCL1, Lane 2: SjCL2, Lane 3: SjCL3, Lane 4: SjCL4, Lane 5: <t>SjCL5.</t>
P Ets2 Thr72, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Recombinant proteins of SjCL1-5 were resolved in 12% SDS-PAGE and stained with Coomassie brilliant blue (A), and analyzed by western blot using a mouse monoclonal antibody against the His-tag (B), or a mixture of serum samples (in equal volume) from 6 S. japonicum -infected BALB/c mice (C) and 5 S. japonicum -infected rabbits (D) collected at 42 dpi, respectively. Lane M: Marker, Lane 1: SjCL1, Lane 2: SjCL2, Lane 3: SjCL3, Lane 4: SjCL4, Lane 5: SjCL5.

Journal: PLOS Neglected Tropical Diseases

Article Title: Schistosoma japonicum cathepsin L1: A potential target for anti-schistosomiasis strategies

doi: 10.1371/journal.pntd.0013241

Figure Lengend Snippet: Recombinant proteins of SjCL1-5 were resolved in 12% SDS-PAGE and stained with Coomassie brilliant blue (A), and analyzed by western blot using a mouse monoclonal antibody against the His-tag (B), or a mixture of serum samples (in equal volume) from 6 S. japonicum -infected BALB/c mice (C) and 5 S. japonicum -infected rabbits (D) collected at 42 dpi, respectively. Lane M: Marker, Lane 1: SjCL1, Lane 2: SjCL2, Lane 3: SjCL3, Lane 4: SjCL4, Lane 5: SjCL5.

Article Snippet: The peptidase_C1 domain fragments of SjCL1 (GeneBank ID: AY814043.1 , 788–1387 nt), SjCL2 ( AY813594.1 , 735–1348 nt), SjCL3 ( FN314782.1 , 370–1017 nt), SjCL4 ( AY222874.2 , 426–1034 nt) and SjCL5 ( FN313884.1 , 441–1070 nt) encoding genes were successfully amplified from schistosome cDNA using high fidelity Phusion DNA polymerase (Finnzymes Oy, Finland).

Techniques: Recombinant, SDS Page, Staining, Western Blot, Infection, Marker

BALB/c mice (n = 10 per group) were immunized with recombinant SjCL1, SjCL2, SjCL3, SjCL4, SjCL5, respectively, or with PBS as control. Following the final immunization, the mice were challenged with cercariae. The blank group (n = 10) consists of mice immunized with PBS but without cercariae challenge. The adult worm burden (A) and liver egg burden (B) were assessed at 42 dpi. for the SjCL1-, SjCL2-, SjCL3-, SjCL4-, SjCL5-immunized groups and PBS control group. The weight of the bodies (C), livers (D) and spleens (E) were measured for all the groups at 42 dpi. Data are presented as mean ± SD. Comparisons were performed between diffident groups with the PBS group, *, p < 0.05; **, p < 0.01; ***, p < 0.0001. The results are representative of two independent experiments.

Journal: PLOS Neglected Tropical Diseases

Article Title: Schistosoma japonicum cathepsin L1: A potential target for anti-schistosomiasis strategies

doi: 10.1371/journal.pntd.0013241

Figure Lengend Snippet: BALB/c mice (n = 10 per group) were immunized with recombinant SjCL1, SjCL2, SjCL3, SjCL4, SjCL5, respectively, or with PBS as control. Following the final immunization, the mice were challenged with cercariae. The blank group (n = 10) consists of mice immunized with PBS but without cercariae challenge. The adult worm burden (A) and liver egg burden (B) were assessed at 42 dpi. for the SjCL1-, SjCL2-, SjCL3-, SjCL4-, SjCL5-immunized groups and PBS control group. The weight of the bodies (C), livers (D) and spleens (E) were measured for all the groups at 42 dpi. Data are presented as mean ± SD. Comparisons were performed between diffident groups with the PBS group, *, p < 0.05; **, p < 0.01; ***, p < 0.0001. The results are representative of two independent experiments.

Article Snippet: The peptidase_C1 domain fragments of SjCL1 (GeneBank ID: AY814043.1 , 788–1387 nt), SjCL2 ( AY813594.1 , 735–1348 nt), SjCL3 ( FN314782.1 , 370–1017 nt), SjCL4 ( AY222874.2 , 426–1034 nt) and SjCL5 ( FN313884.1 , 441–1070 nt) encoding genes were successfully amplified from schistosome cDNA using high fidelity Phusion DNA polymerase (Finnzymes Oy, Finland).

Techniques: Recombinant, Control